Kodin   


Determination of the speed of bioremedation in absorbents using petroleum products.

According to the contract between two sides, the purpose of current work is to examine the speed of biodegradation of petroleum product in hardly polluted hydrophobic absorbent.

For the test JORESIN OY provided six (6) different samples in plastic bags, that contained absorbents polluted with petroleum products and textile with polysaccharose in equal amounts. The weight of entire test material was 148,72 g, from which 79,07 g was textile and 69,72-g absorbent. According to the clients request, the test where curried out in the style of average sample. For that purpose all samples where mixed together.

Referring to the literature materials (1-4) and experience of laboratory, it was assumed that the process of biodegradation in hydrophobic environment is slow. The lab decided to perform parallel test, in that case the accelerator for biodegradation ”Hydrobreak Plus”  @ 1% was added to the test.

Experimental Method

Tests where carried out in an isolated environment, where there was a guaranteed replacement of carbon dioxide with oxygen, in order to maintain the constant pressure for oxygen during the entire test. The temperature during the test was 20+/-2 C.

Test material was located in the environment of saturated water vapor during the entire test period. During the test the volume of petroleum products was determined once a month in the form of share tests in the method of abstract (3g) from textile and from absorbents. The content of petroleum products in the share test was determined with the infra red spectrographometer and in addition in August and October with gas chromatography.

                                                Results

Results of analyses (mg petroleum products in 1g sample) are given in the table and presented in graph:

Dates when analyses

In absorbent

In textile

Absorbent +

Hydrobreak

Textile +

Hydrobreak

 

05.05

180

178

180

179

05.06

165

162

120

125

06.07

125

115

85

80

05.08

110

95

60

55

07.09

93

75

51

48

05.10

81

69

47

40

  

The containing of petroleum products in absorbent and in textile

                                          The date  

Results

 

1.   The biodegradation of petroleum products in the examined tests takes place slowly. The supplement Hydrobreak Plus speeded up the process of biodegradation noticeably.

2.   The main reason behind low speed of biodegradation is the high hydrophobic environment. Adding the hydrophilic substance Hydrobreak Plus speeded up the process of degradation essentially.

3.   In order to speed up the process of biodegradation it is advised to add the component of hydrophilic to the environment, which can be; sawdust smashed bark of tree, mill peat, soil etc. The relationship between materials added and sorbs may be 1: 1.

4.   During the trial period in the visual observation degrades more then half of polysaharos thin layer.

5.   All recommendations given above are in the qualitative nature and demand optimizing.

Literature

1. Contaminated Soil ’98, Proceedings of the Sixth International FKZ/TNO Conference on Contaminated Soil, 17-21 May 1998, Edinburgh, UK, Volumes I, II.

 

 

     

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